Purification and characterisation of antigenic gliadins in coeliac disease
1992; Elsevier BV; Volume: 207; Issue: 3 Linguagem: Inglês
10.1016/0009-8981(92)90121-6
ISSN1873-3492
AutoresHans Sjöström, S. Friis, Ove Norén, Dorit Anthonsen,
Tópico(s)Monoclonal and Polyclonal Antibodies Research
ResumoTwo gliadins, known to be especially antigenic in coeliac disease, were purified to homogeneity by a series of ion-exchange chromatography steps. Their N-terminal amino acid sequences showed minor differences but clearly classified them as gamma-type gliadins. The purified gliadins were further characterised with respect to amino acid composition, molecular mass and E1(1%)cm at 276 nm. Based on these properties it is suggested that one of them is identical to a gamma-type gliadin, earlier characterised by its nucleotide sequence, whereas the other has not previously been described. The purification procedure may form the basis for the development of a more differentiated analysis of circulating antibodies for diagnosis and makes clinical testing of the toxicity of defined gliadin peptides feasible.
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