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In vitro maturation of mouse oocytes isolated from late, middle, and pre‐antral graafian follicles

1974; Wiley; Volume: 190; Issue: 1 Linguagem: Inglês

10.1002/jez.1401900112

1097-010X

Gregory F. Erickson, Ralph A. Sorensen,

Ovarian function and disorders

Abstract Oocytes were dissected from late, middle, and pre‐antral Graafian follicles from sexually mature mice (6–8 weeks old), cultured in vitro in a chemically defined medium for 18 hours, and scored for meiotic maturation. Between 83–91% of the oocytes removed from antral follicles (300–600 μm in diameter) progressed to either metaphase I or metaphase II. In marked contrast, 98% of the oocytes removed from pre‐antral follicles (100–150 μm in diameter) failed to resume meiosis and remained in the germinal vesicle stage. These and other data suggest there is a close correlation between the capacity of the mouse oocyte to resume meiosis and the appearance of the antrum in the developing follicle.