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Insight in eukaryotic ABC transporter function by mutation analysis

2006; Wiley; Volume: 580; Issue: 4 Linguagem: Inglês

10.1016/j.febslet.2006.01.024

1873-3468

Annie Frelet‐Barrand, Markus Klein,

Amino Acid Enzymes and Metabolism

With regard to structure–function relations of ATP‐binding cassette (ABC) transporters several intriguing questions are in the spotlight of active research: Why do functional ABC transporters possess two ATP binding and hydrolysis domains together with two ABC signatures and to what extent are the individual nucleotide‐binding domains independent or interacting? Where is the substrate‐binding site and how is ATP hydrolysis functionally coupled to the transport process itself? Although much progress has been made in the elucidation of the three‐dimensional structures of ABC transporters in the last years by several crystallographic studies including novel models for the nucleotide hydrolysis and translocation catalysis, site‐directed mutagenesis as well as the identification of natural mutations is still a major tool to evaluate effects of individual amino acids on the overall function of ABC transporters. Apart from alterations in characteristic sequence such as Walker A, Walker B and the ABC signature other parts of ABC proteins were subject to detailed mutagenesis studies including the substrate‐binding site or the regulatory domain of CFTR. In this review, we will give a detailed overview of the mutation analysis reported for selected ABC transporters of the ABCB and ABCC subfamilies, namely HsCFTR/ABCC7, HsSUR/ABCC8,9, HsMRP1/ABCC1, HsMRP2/ABCC2, ScYCF1 and P‐glycoprotein (Pgp)/MDR1/ABCB1 and their effects on the function of each protein.