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Pulling Genetic RNA out of Tobacco Mosaic Virus Using Single-Molecule Force Spectroscopy

2010; American Chemical Society; Volume: 132; Issue: 32 Linguagem: Inglês

10.1021/ja1052544

1943-2984

Ningning Liu, Bo Peng, Yuan Lin, Zhaohui Su, Zhongwei Niu, Qian Wang, Wenke Zhang, Hongbin Li, Jiacong Shen,

Mechanical and Optical Resonators

RNA−coat protein interactions in intact tobacco mosaic virus have been investigated for the first time directly on the single-molecule level by pulling the genetic RNA step by step out of the helical groove formed by its protein coat. The effects of pulling speed and pH on RNA−protein interactions are presented. In addition, the rebinding behavior of the detached RNA with the protein coat is discussed. Our results demonstrate the possibility of studying nucleic acid−protein interactions in more complicated systems using AFM-based single-molecule force spectroscopy.