Housekeeping Proteins: Limitations as References During Neuronal Differentiation
2008; Bentham Science Publishers; Volume: 2; Issue: 1 Linguagem: Inglês
10.2174/1874082000802010036
ISSN1874-0820
Autores Tópico(s)Pluripotent Stem Cells Research
ResumoThe use of "Housekeeping proteins" as loading controls in western blots requires that they do not change their level of expression.However, deviations from this rule have been observed.This study investigated the expression of the commonly used proteins HSP60, -tubulin, -actin and GAPDH by western blot during retinoic acid-induced differentiation of the embryonal carcinoma NTera-2 and the neuroblastoma SH-SY5Y cell lines.-actin was found to be a poor loading control, increasing significantly during differentiation, whereas HSP60 and GAPDH were the more evenly expressed of those analysed.Our results underline the importance of selecting accurate loading controls in this biological context.
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