Derivatization in Mass Spectrometry—8. Soft Ionization Mass Spectrometry of Small Molecules
2006; SAGE Publishing; Volume: 12; Issue: 2 Linguagem: Inglês
10.1255/ejms.798
ISSN1751-6838
Autores Tópico(s)Metabolomics and Mass Spectrometry Studies
ResumoThis is the first of two reviews devoted to derivatization approaches for “soft” ionization mass spectrometry (fast atom bombardment, matrix assisted laser desorption/ionization, electrospray ionization and atmospheric pressure chemical ionization) and deals, in particular, with small molecules. The principles of the main “soft” ionization mass spectrometric methods as well as the reasons for derivatizing small molecules are briefly described. Derivatization methods for modification of amines, carboxylic acids, amino acids, alcohols, carbonyl compounds, monosaccharides, thiols, unsaturated and aromatic compounds etc. to improve their ionizability and to enhance structure information content are discussed. The use of “fixed”-charge bearing derivatization reagents is especially emphasized. Chemical aspects of derivatization and “soft” ionization mass spectrometric properties of derivatives are considered.
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