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Crystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia gladiata seeds

2004; Wiley; Volume: 60; Issue: 8 Linguagem: Inglês

10.1107/s0907444904014489

1399-0047

Frederico Bruno Mendes Batista Moreno, P. Delatorre, Beatriz Tupinambá Freitas, B.A.M. Rocha, Emmanuel Prata de Souza, Felipe Facó, Fernanda Canduri, André Herzog Cardoso, V. N. Freire, José Luiz Lima Filho, Alexandre Holanda Sampaio, Juan J. Calvete, Walter Filgueira de Azevedo, Benildo Sousa Cavada,

Carbohydrate Chemistry and Synthesis

The seed lectin from Canavalia gladiata was purified and crystallized. Orthorhombic crystals belonging to space group C2221 grew within three weeks at 293 K using the hanging-drop vapour-diffusion method. Using synchrotron X-ray radiation, a complete structural data set was collected at 2.3 Å resolution. The preliminary crystal structure of the lectin, determined by molecular replacement, had a correlation coefficient of 0.569 and an R factor of 0.412.