Ag@Poly( m -phenylenediamine) Core−Shell Nanoparticles for Highly Selective, Multiplex Nucleic Acid Detection
2011; American Chemical Society; Volume: 27; Issue: 6 Linguagem: Inglês
10.1021/la105092f
ISSN1520-5827
AutoresYingwei Zhang, Lei Wang, Jingqi Tian, Hailong Li, Yonglan Luo, Xuping Sun,
Tópico(s)Nanocluster Synthesis and Applications
ResumoIn this letter, we report on the one-step synthesis of Ag@poly(m-phenylenediamine) core−shell nanoparticles (APCSNPs), carried out by direct mixing of aqueous silver nitrate and m-phenylenediamine solutions at room temperature. We further demonstrate the use of APCSNP as a novel fluorescent sensing platform for nucleic acid detection. In this regard, the detection of DNA is accomplished in two steps. First, APCSNP absorbs and quenches the fluorescence of dye-labeled single-stranded DNA (ssDNA) as a probe. Second, hybridizing of the probe with its target produces a double-stranded DNA (dsDNA) that detaches from APCSNP, resulting in the recovery of dye fluorescence. It suggests that this sensing system has a high selectivity down to single-base mismatch, and the results exhibit good reproducibility. Furthermore, we also demonstrate its application for the multiplex detection of nucleic acid sequences.
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