Transfer of amino acids between neuroglia cells and neurons in the leech ganglion
1973; Elsevier BV; Volume: 40; Issue: 1 Linguagem: Inglês
10.1016/0014-4886(73)90127-1
ISSN1090-2430
AutoresAlbert Globus, H. D. Lux, Peter Schubert,
Tópico(s)Olfactory and Sensory Function Studies
ResumoIntracellular iontophoretic injection of [3H]- amino acids into glia and neurons of the isolated leech ganglion (Hirudo medicinalis) and subsequent autoradiography is useful for studying neuroglia-neuron interactions and, in particular, the transfer of amino acids or metabolites from neuroglia cells to neurons and between neurons. By means of a three-barreled glass electrode either nerve and neuroglia cells were impaled under direct vision. After electrophysiological identification of these cells, tritiated leucine or glycine were intracellularly applied using an iontophoresis current of 10−8–10−7 amp. The amino acids were found incorporated within the nerve cell soma and transported into the axon. A transfer of amino acids from neuroglia to nerve cells was confirmed. There are observations which suggest a special exchange of amino acids or metabolites between the electrically coupled Retzius cells.
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