Voltar
Artigo Produção Nacional
BIBTEX RIS

Copper ions mediate the lethality induced by hydrogen peroxide in low iron conditions in Escherichia coli

2000; Elsevier BV; Volume: 460; Issue: 1 Linguagem: Inglês

10.1016/s0921-8777(00)00014-8

ISSN

1386-1476

Autores

Carlos Eduardo Bonacossa de Almeida, Rodrigo S. Galhardo, Deise L. Felı́cio, Januário B. Cabral‐Neto, Álvaro C. Leitão,

Tópico(s)

Metal complexes synthesis and properties

Resumo

Iron ions mediate the formation of lethal DNA damage by hydrogen peroxide. However, when cells are depleted of iron ions by the treatment with iron chelators, DNA damage can still be detected. Here we show that the formation of such damage in low iron conditions is due to the participation of copper ions. Copper chelators can inhibit cell inactivation, DNA strand breakage and mutagenesis induced by hydrogen peroxide in cells pre-treated with iron chelators. The Fpg and UvrA proteins play an important role in the repair of DNA lesions formed in these conditions, as suggested by the great sensitivity of the uvrA and fpg mutant strains to the treatment when compared to the wild type strain.

Referência(s)