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P-selectin (CD62) binds to subpopulations of human memory T lymphocytes and natural killer cells

1992; Elsevier BV; Volume: 186; Issue: 1 Linguagem: Inglês

10.1016/s0006-291x(05)80790-9

1090-2104

Kevin L. Moore, Linda F. Thompson,

Atherosclerosis and Cardiovascular Diseases

P-selectin (CD62) is a Ca2+-dependent lectin expressed on activated platelets and endothelium. Although P-selectin is known to function as a receptor for myeloid cells, previous studies indicated that P-selectin also bound to a subset of lymphocytes. Using a multi-color immunofluorescence assay we found that purified P-selectin bound to 12.2 ± 4.1 % of peripheral blood lymphocytes and that P-selectin could mediate adhesion of activated platelets to lymphocytes. A subpopulation of CD4+, CD8+, and CD16+ lymphocytes bound P-selectin. There was a marked preference for P-selectin binding to memory cells (CD45RO+) in both the CD4+ and CD8+ populations. Binding to all cell types was Ca2+-dependent and blocked by pretreatment of the cells with sialidase. These data suggest that P-selectin may play a role in the recruitment of specific lymphocyte populations to sites of inflammation.