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Partial protective responses induced by a recombinant cysteine proteinase from Leishmania (Leishmania) amazonensis in a murine model of cutaneous leishmaniasis

2009; Elsevier BV; Volume: 124; Issue: 2 Linguagem: Inglês

10.1016/j.exppara.2009.09.003

1090-2449

Carlos Eduardo Cardoso Fedeli, Josie Haydée Lima Ferreira, Juliana Sekeres Mussalem, Ieda Maria Longo‐Maugéri, Luciana Girotto Gentil, Márcia Regina Machado dos Santos, Simone Katz, C. Barbieri,

Blood Coagulation and Thrombosis Mechanisms

A 500 bp fragment encoding an isoform of cysteine proteinase from Leishmania (Leishmania) amazonensis was subcloned and expressed in the pHis vector, resulting in a recombinant protein of 24 kDa, rLacys24. In Western blots of L. (L.) amazonensis extracts, antibodies directed to rLacys24 recognized a cysteine proteinase isoform of 30 kDa. Analysis by fluorescence-activated cell sorter showed a significantly higher expression of CD8(+) lymphocytes in animals immunized with rLacys24 plus CFA, whereas a low expression of CD4(+) lymphocytes was observed in these animals. The cytotoxicity of lymphocytes isolated from mice immunized with rLacys24 plus CFA on L. (L.) amazonensis-infected macrophages was significantly higher than that observed in the presence of lymphocytes from control animals. Immunization of BALB/c mice with rLacys24 plus CFA resulted in a low but significant decrease of foot lesions after challenge with L. (L.) amazonensis compared to those exhibited by control mice.