MIXED CULTURE CYTOPATHOGENICITY: A NEW TEST FOR GROWTH OF MURINE LEUKEMIA VIRUSES IN TISSUE CULTURE
1969; National Academy of Sciences; Volume: 63; Issue: 3 Linguagem: Inglês
10.1073/pnas.63.3.753
ISSN1091-6490
AutoresVáclav Klement, Wallace P. Rowe, Janet W. Hartley, Wendell E. Pugh,
Tópico(s)Molecular Biology Techniques and Applications
ResumoTissue culture cells of a Rous virus-induced rat tumor undergo syncytium formation when placed in contact with mouse embryo cell cultures infected with murine leukemia viruses. This phenomenon can be used as a cytopathic end point for isolating and titrating these viruses in tissue culture. The principle should be applicable to detection of leukemia viruses of other species.
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