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Phenotype-Dependent Expression of α-Smooth Muscle Actin in Visceral Smooth Muscle Cells

1999; Elsevier BV; Volume: 247; Issue: 1 Linguagem: Inglês

10.1006/excr.1998.4339

1090-2422

Hiroshi Saga, Kazuhiro Kimura, Ken’ichiro Hayashi, Takahiro Gotow, Yasuo Uchiyama, Takuya MOMIYAMA, S Tadokoro, Nozomu Kawashima, Akie Jimbou, Kenji Sobue,

Protease and Inhibitor Mechanisms

Alpha-Smooth muscle actin is one of the molecular markers for a phenotype of vascular smooth muscle cells, because the actin is a major isoform expressed in vascular smooth muscle cells and its expression is upregulated during differentiation. Here, we first demonstrate that the phenotype-dependent expression of this actin in visceral smooth muscles is quite opposite to that in vascular smooth muscles. This actin isoform is not expressed in adult chicken visceral smooth muscles including gizzard, trachea, and intestine except for the inner layer of intestinal muscle layers, whereas its expression is clearly detected in these visceral smooth muscles at early stages of the embryo (10-day-old embryo) and is developmentally downregulated. In cultured gizzard smooth muscle cells maintaining a differentiated phenotype, alpha-smooth muscle actin is not detected while its expression dramatically increases during serum-induced dedifferentiation. Promoter analysis reveals that a sequence (-238 to -219) in the promoter region of this actin gene acts as a novel negative cis-element. In conclusion, the phenotype-dependent expression of alpha-smooth muscle actin would be regulated by the sum of the cooperative contributions of the negative element and well-characterized positive elements, purine-rich motif, and CArG boxes and their respective transacting factors.