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Immunopontentiating and antitumor activities of a polysaccharide from Pulsatilla chinensis (Bunge) Regel

2012; Elsevier BV; Volume: 54; Linguagem: Inglês

10.1016/j.ijbiomac.2012.12.012

1879-0003

Tong Liu, Leiguang Ye, Xueqing Guan, Xiaoshuan Liang, Cong Li, Qian Sun, Yang Liu, Shiru Chen, Fa Bang, Baogang Liu,

Fungal Biology and Applications

One water-soluble polysaccharide (PCPw) was isolated and purified from the roots of Pulsatilla chinensis by DEAE cellulose-52 and Sephadex G-100 column chromatography, and its antitumor activity was evaluated on 4T1 tumor-bearing mice through transplantable animal tumor. After 10 days of PCPw (50, 100 and 200 mg/kg) treatment once daily in tumor-bearing mice, PCPw oral administration could not only significantly inhibit the growth of transplantable 4T1 tumor in mice but also promote concanavalin A (Con A), lipopolysaccharide (LPS)-stimulated splenocytes proliferation, the serum lysozyme level and 2,4-dinitrofluorobenzene (DNFB)-induced delayed-type hypersensitivity (DTH) reactions, especially at the dose of 100 mg/kg. Meanwhile, significant improvements in peripheral blood abnormality and anemia were observed in PCPw-treated group. These results suggested that PCPw could improve both cellular and humoral immune response and might be explored as a potential natural antitumor drug.