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A New Enzymatic Method for the Determination of Inulin

1995; SAGE Publishing; Volume: 32; Issue: 6 Linguagem: Inglês

10.1177/000456329503200608

1758-1001

Osamu Sugita, Yoshiaki Tomiyama, Takayuki Matsuto, Masaki Okada, Fumitake Gejyo, Masaaki Arakawa, Saori Takahashi, Y Watazu, N. Kaneda,

Microbial Metabolites in Food Biotechnology

A new enzymatic method for the determination of inulin in plasma and urine, using inulase (EC 3.2.1.7), fructokinase (EC 2.7.1.4), phosphoglucoisomerase (EC 5.3.1.9) and glucose-6-phosphate dehydrogenase (EC 1.1.1.49) is described. The assay is based on the hydrolysis of inulin or Inutest (INutest which is the injectable form of inulin), by inulase and the determination of fructose released. The assay was linear up to 2 g/L of Inutest. The within-batch and between batch coefficients of variation were 2.3% and 2.2%, respectively. Recovery of added Inutest from plasma and urine was 98-102%. There was no interference from glucose (27.7 mmol/L), fructose (1.7 mmol/L) or mannose (1.7 mmol/L). When inulin clearance (using this method) and thiosulphate clearance were compared in 37 patients the inulin clearance was 9.3 mL/min (12%) lower than the thiosulphate clearance. We conclude that this enzymatic method is a simple and specific method.