Protein synthesis in plant mitochondria☆III. Characterization of mitochondria and the microsomal fractionm of the seedlings of vigna sinensis
1964; Elsevier BV; Volume: 93; Issue: 2 Linguagem: Inglês
10.1016/0304-4165(64)90379-4
ISSN1872-8006
AutoresHirendra Das, Tanmoy Mukherjee,
Tópico(s)Genetic and Environmental Crop Studies
ResumoAbstract 1. 1. Glucose-6-phosphatase (EC 3.1.3.9) activity was considerable in the microsomal fraction but was absent in the mitochondria cell from Vigna sinesis. Ribonucleic acid in the microsomal fraction was sensitive to exogenous ribonuclease (EC 2.7.7.16) while that in mitochondria remained unaffected. 2. 2. Electron-microscopy of the microsomal fraction revealed prepoderance of particle (110–160 A in diameter), with relatively rare occurrence of membranes, polyribosome-like structures and other small particles (60–70 A) of considerably greater electron density. 3. 3. Electron-microscopy of the mitochondrial pellet revealed typical structures of mitochondria with different degrees of preservation. Ribosomes, whole cells or nuclei were absent. Mitochondia in the “five-times-washed” pellets had greater number of well preserved cristae and less heterogenous matrix than those in the “once-washed” pellets. 4. 4. These observations have been discussed in the light of the earlier finding that mitochondria were more active than the microsomal fraction in incorporating amino acid into their protein.
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