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Genetic Characterization of 2 Novel Feline Caliciviruses Isolated from Cats with Idiopathic Lower Urinary Tract Disease

2002; Wiley; Volume: 16; Issue: 3 Linguagem: Inglês

10.1892/0891-6640(2002)016 2.3.co;2

1939-1676

Cheryl C. Rice, John M. Kruger, Patrick J. Venta, Aivars Vilnis, Kara A. Maas, Jennifer A. Dulin, Roger K. Maes,

Herpesvirus Infections and Treatments

Journal of Veterinary Internal MedicineVolume 16, Issue 3 p. 293-302 Open Access Genetic Characterization of 2 Novel Feline Caliciviruses Isolated from Cats with Idiopathic Lower Urinary Tract Disease Cheryl C. Rice, Cheryl C. Rice Departments of Small Animal Clinical Sciences Michigan State University College of Veterinary Medicine, East Lansing, MI.Search for more papers by this authorJohn M. Kruger, Corresponding Author John M. Kruger Departments of Small Animal Clinical Sciences Michigan State University College of Veterinary Medicine, East Lansing, MI. Department of Small Animal Clinical Sciences, Room D208 Veterinary Clinical Center, Michigan State University, East Lansing, MI 48824–1314; e-mail: [email protected] msu. edu.Search for more papers by this authorPatrick J. Venta, Patrick J. Venta Departments of Small Animal Clinical Sciences Michigan State University College of Veterinary Medicine, East Lansing, MI. Departments of Microbiology Michigan State University College of Veterinary Medicine, East Lansing, MI.Search for more papers by this authorAivars Vilnis, Aivars Vilnis Departments of Microbiology Michigan State University College of Veterinary Medicine, East Lansing, MI.Search for more papers by this authorKara A. Maas, Kara A. Maas Departments of Small Animal Clinical Sciences Michigan State University College of Veterinary Medicine, East Lansing, MI.Search for more papers by this authorJennifer A. Dulin, Jennifer A. Dulin Departments of Small Animal Clinical Sciences Michigan State University College of Veterinary Medicine, East Lansing, MI.Search for more papers by this authorRoger K. Maes, Roger K. Maes Departments of Microbiology Michigan State University College of Veterinary Medicine, East Lansing, MI. Departments of Animal Health Diagnostic Laboratory, Michigan State University College of Veterinary Medicine, East Lansing, MISearch for more papers by this author Cheryl C. Rice, Cheryl C. Rice Departments of Small Animal Clinical Sciences Michigan State University College of Veterinary Medicine, East Lansing, MI.Search for more papers by this authorJohn M. Kruger, Corresponding Author John M. Kruger Departments of Small Animal Clinical Sciences Michigan State University College of Veterinary Medicine, East Lansing, MI. Department of Small Animal Clinical Sciences, Room D208 Veterinary Clinical Center, Michigan State University, East Lansing, MI 48824–1314; e-mail: [email protected] msu. edu.Search for more papers by this authorPatrick J. Venta, Patrick J. Venta Departments of Small Animal Clinical Sciences Michigan State University College of Veterinary Medicine, East Lansing, MI. Departments of Microbiology Michigan State University College of Veterinary Medicine, East Lansing, MI.Search for more papers by this authorAivars Vilnis, Aivars Vilnis Departments of Microbiology Michigan State University College of Veterinary Medicine, East Lansing, MI.Search for more papers by this authorKara A. Maas, Kara A. Maas Departments of Small Animal Clinical Sciences Michigan State University College of Veterinary Medicine, East Lansing, MI.Search for more papers by this authorJennifer A. Dulin, Jennifer A. Dulin Departments of Small Animal Clinical Sciences Michigan State University College of Veterinary Medicine, East Lansing, MI.Search for more papers by this authorRoger K. Maes, Roger K. Maes Departments of Microbiology Michigan State University College of Veterinary Medicine, East Lansing, MI. Departments of Animal Health Diagnostic Laboratory, Michigan State University College of Veterinary Medicine, East Lansing, MISearch for more papers by this author First published: 28 June 2008 https://doi.org/10.1111/j.1939-1676.2002.tb02372.xCitations: 17AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Feline caliciviruses (FCVs) are potential etiologic agents in feline idiopathic lower urinary tract disease (I-LUTD). By means of a modified virus isolation method, we examined urine obtained from 28 male and female cats with nonobstructive I-LUTD, 12 male cats with obstructive I-LUTD, and 18 clinically healthy male and female cats. All cats had been routinely vaccinated for FCV. Two FCVs were isolated; 1 (FCV-U1) from a female cat with nonobstructive I-LUTD, and another (FCV-U2) from a male cat with obstructive I-LUTD. To determine the genetic relationship of FCV-U1 and FCV-U2 to other FCVs, capsid protein gene RNA was reverse transcribed into cDNA, amplified, and sequenced. Multiple amino acid sequence alignments and phylogenetic trees were constructed for the entire capsid protein, hypervariable region E, and the more conserved (nonhypervariable) regions A, B, D, and F. When compared to 23 other FCV isolates with known biotypes, the overall amino acid sequence identity of the capsid protein of FCV-U1 and FCV-U2 ranged from 83 to 96%; identity of hypervariable regions C and E ranged from 58 to 85%. Phylogenetically, FCV-U1 clearly separated from other FCV strains in phenograms based on nonhypervariable regions. In contrast, FCV-U2 consistently segregated with the Urbana strain in all phenograms. Clustering of isolates by geographic origin was most apparent in phenograms based on nonhypervariable regions. No clustering of isolates by biotype was apparent in any phenograms. Our results indicate that FCV-U1 and FCV-U2 are genetically distinct from other known vaccine and field strains of FCV. References 1 Osborne CA, Kruger JM, Lulich JP, Polzin DJ. Feline lower urinary tract disorders. In: SJ Ettinger, ed. Textbook of Veterinary Internal Medicine, 4th ed. Philadelphia , PA : WB Saunders; 1995: 1805– 1832. 2 Buffington CAT, Chew DJ, Kendall MS, et al. Clinical evaluation of cats with nonobstructive urinary tract diseases. J Am Vet Med Assoc 1997; 210: 46– 50. 3 Kruger JM, Osborne CA, Goyal SM. Clinical evaluation of cats with lower urinary tract disease. J Am Vet Med Assoc 1991; 199: 211– 216. 4 Kalkstein TS, Kruger JM, Osborne CA. Feline idiopathic lower urinary tract disease. Part 2: Potential causes. Compend Cont Educ Pract Vet 1999; 21: 148– 154. 5 Kruger JM, Osborne CA, Venta PJ, et al. Viral infections of the feline urinary tract. Vet Clin North Am Small Anim Pract 1996; 26: 281– 296. 6 Fabricant CG. Herpes-virus-induced urolithiasis in specific pathogen free male cats. Am J Vet Res 1977; 38: 1837– 1842. 7 Fabricant CG. The feline urological syndrome induced by injection with a cell-associated herpesvirus. Vet Clin North Am Small Anim Pract 1984; 14: 493– 502. 8 Rich LJ, Fabricant CG, Gillespie JH. Urethral obstruction in male cats: Transmission studies. Can J Comp Med 1969; 33: 164– 165. 9 Rich LJ, Fabricant CG, Gillespie JH. Virus induced urolithiasis in male cats. Cornell Vet 1971; 61: 542– 553. 10 Kruger JM, Osborne CA. The role of viruses in feline lower urinary tract disease. J Vet Intern Med 1990; 4: 71– 78. 11 Komolafe OO. Effect of storage on the integrity of purified calicivirus particles. Microbios 1979; 26: 137– 146. 12 Kalkstein TS, Kruger JM, Osborne CA. Feline idiopathic lower urinary tract disease. Part 1: Clinical manifestations. Compend Cont Educ Pract Vet 1999; 21: 15– 26. 13 Crandall RA, Madin SH. Experimental studies on a new feline virus. Am J Vet Res 1960; 21: 551– 556. 14 Gibbs EP, Smale CJ, Voyle CA. Electron microscopy as an aid to the rapid diagnosis of virus diseases of veterinary importance. Vet Rec 1980; 106: 451– 458. 15 Carter MJ, Milton ID, Meanger J, et al. The complete nucleo-tide sequence of feline calicivirus. Virology 1992; 190: 442– 448. 16 Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor , NY : Cold Spring Harbor Laboratory Press; 1989: 5.28– 5.32. 17 Voss H, Schwager C, Wiemanns S, et al. Efficient low redundance large scale DNA sequencing at EMBL. J Biotechnol 1995; 41: 121– 129. 18 Dulbecco R, Vogt M. Plaque formation and isolation of pure line with poliomyelitis viruses. J Exp Med 1954; 99: 167– 182. 19 Baulch-Brown C, Love D, Meanger J. Sequence variation within the capsid protein of Australian isolates of feline calicivirus. Vet Microbiol 1999; 68: 107– 117. 20 Glenn M, Radford AD, Turner PC, et al. Nucleotide sequence of UK and Australian isolates of feline calicivirus (FCV) and phylo-genetic analysis of FCV's. Vet Microbiol 1999; 67: 175– 193. 21 Van Vuuren M, Geissler K, Gerber D, et al. Characterization of a potentially abortigenic strain of calicivirus isolated from a domestic cat. Vet Rec 1999; 144: 636– 638. 22 Geissler K, Schneider, Platzer G, et al. Genetic and antigenic heterogeneity among feline calicivirus isolates from distinct disease manifestations. Virol Res 1997; 48: 193– 206. 23 Sonovtsev S, Green KY. RNA transcripts derived from a cloned full-length copy of the feline calicivirus genome do not require VpG for infectivity. Virology 1995; 210: 383– 390. 24 Pedersen NC, Laliberte L, Ekman S. A transient febrile "limping" syndrome of kittens caused by two different strains of feline calicivirus. Feline Pract 1983; 13: 26– 35. 25 Seal BS, Neill JD. Capsid protein gene sequence of feline calicivirus isolates 255 and LLK: Further evidence for capsid protein configuration among feline caliciviruses. Virus Genes 1995; 9: 183– 187. 26 Seal BS, Ridpath JF, Mengeling WL. Analysis of feline calicivirus capsid protein genes: Identification of variable antigenic determinant regions of the protein. J Gen Virol 1993; 74: 2519– 2524. 27 Fastier LB. A new feline virus isolated in tissue culture. Am J Vet Res 1957; 18: 382– 389. 28 Bittle JL, York CJ, Newberne JW, et al. Serologic relationship of new feline cytopathogenic viruses. Am J Vet Res 1960; 21: 547– 550. 29 Kahn DE, Gillespie JH. Feline viruses. X. Characterization of a newly-isolated picornavirus causing interstitial pneumonia and ul-cerative stomatitis in the domestic cat. Cornell Vet 1970; 60: 669– 683. 30 Seal BS. Analysis of capsid protein variation among divergent isolates of feline calicivirus. Virus Res 1994; 33: 39– 53. 31 Takahashi E, Konishi S, Ogata M. Studies on cytopathogenic viruses from cats with respiratory infections II. Characterization of feline picornaviruses. Jpn J Vet Sci 1971; 33: 81– 87. 32 Tohya Y, Taniguchi Y, Takahashi E, et al. Sequence analysis of the ′-end of feline calicivirus genome. Virology 1991; 183: 810– 814. 33 Oshikama R, Tohya Y, Kawaguchi Y, et al. The molecular cloning and sequence of an open reading frame encoding for nonstructural proteins of feline calicivirus F4 strain isolated from Japan. J Vet Med Sci 1994; 56: 1093– 1099. 34 Neill JD, Reardon IM, Heinrikson RL. Nucleotide sequence and expression of the capsid protein gene of feline calicivirus. J Virol 1991; 65: 5440– 5447. 35 Matson DO, Berke T, Dinulos MB, et al. Partial characterization of the genome of nine animal caliciviruses. Arch Virol 1996; 141: 2443– 2456. 36 Fredricks DN, Relman DA. Sequence-based identification of microbial pathogens: A reconsideration of Koch's postulates. Clin Microbiol Rev 1996; 9: 18– 33. 37 Kreutz LC, Johnson RP, Seal BS. Phenotypic and genotypic variation of feline calicivirus during persistent infection of cats. Vet Microbiol 1998; 59: 229– 236. 38 Radford AD, Turner PC, Bennett M, et al. Quasispecies evolution of a hypervariable region of the feline calicivirus capsid gene in cell culture and in persistently infected cats. J Gen Virol 1998; 79: 1– 10. 39 Guiver M, Littler E, Caul EO, et al. The cloning, sequencing and expression of a major antigenic region from the feline calicivirus capsid protein. J Gen Virol 1992; 73: 2429– 2433. 40 Milton ID, Turner J, Teelan A, et al. Location of monoclonal antibody binding sites in the capsid protein of feline calicivirus. J Gen Virol 1992; 73: 2435– 2439. 41 Tohya Y, Yokoyama N, Maeda K, et al. Mapping of antigenic sites involved in neutralization on the capsid protein of feline calicivirus. J Gen Virol 1997; 78: 303– 305. 42 Radford AD, Bennett M, McArdle F, et al. The use of sequence analysis of a feline calicivirus (FCV) hypervariable region in the ep-idemiological investigation of FCV related disease and vaccine failures. Vaccine 1997; 15: 1451– 1458. 43 Rossman MG, Rueckert RR. What does the molecular structure of viruses tell us about viral functions Microbiol Sci 1987; 4: 206– 214. 44 Sykes JE, Studdert VP, Browning GF. Detection and strain differentiation of feline calicivirus in conjunctival swabs by RT-PCR of the hypervariable region of the capsid protein gene. Arch Virol 1998; 143: 1321– 1334. 45 Carter MJ, Cubitt WD. Norwalk and related viruses. Curr Opin Infect Dis 1995; 8: 403– 409. 46 Le Gall G, Arnauld C, Boilletot E, et al. Molecular epidemiology of rabbit haemorrhagic disease virus outbreaks in France during 1988–1995. J Gen Virol 1998; 79: 11– 16. 47 Leitner T, Escanilla D, Franzen C, et al. Accurate reconstruction of a known HIV-1 transmission history by phylogenetic tree analysis. Proc Natl Acad Sci USA 1996; 93: 10864– 10869. 48 Schneider-Schaulies J. Cellular receptors for viruses: links to tropism and pathogenesis. J Gen Virol 2000; 81: 1413– 1429. 49 Koch R. Ueber bakteriologische Forschung. In: Verhandlungen X Internationalen Medichinischen Congresses, Berlin, 1890. Berlin : August Hirschwald; 1891: 35. 50 Evans AS. Causation and disease: The Henle-Koch postulates revisited. Yale J Biol Med 1976; 49: 175– 195. 51 Rivers TM. Viruses and Koch's postulates. J Bacteriol 1937; 33: 1– 12. Citing Literature Volume16, Issue3May 2002Pages 293-302 ReferencesRelatedInformation