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Detection of Salmonella Typhimurium in Raw Meats using In‐House Prepared Monoclonal Antibody Coated Magnetic Beads and PCR Assay of the fimA Gene

2007; Taylor & Francis; Volume: 29; Issue: 1 Linguagem: Inglês

10.1080/15321810701735096

1532-4230

Ângela Nunes Moreira, Fabrício Rochedo Conceição, Rita de Cássia S. Conceição, Roberta Juliano Ramos, José Beiro Carvalhal, Odir Antônio Dellagostin, José A. G. Aleixo,

Vibrio bacteria research studies

Abstract Abstract A method for detection of Salmonella Typhimurium in meat samples that uses in‐house monoclonal antibody (MAb) coated magnetic beads for immunomagnetic separation (IMS) associated with PCR amplification of the gene fimA was developed. An internal amplification control (IAC) of the PCR reaction was constructed. The fimA PCR has shown 100% sensitivity and specificity when tested with various bacteria. The detection limit of the IMS‐PCR method, using a post‐enrichment in BHI broth for 6 h between IMS and PCR, was 1–10 CFU/mL. The method proved to be rapid (27 hrs), highly sensitive (1–10 CFU/25 g), and specific for detection of S. Typhimurium from experimentally contaminated pork and chicken meat samples. Keywords: Salmonella FoodsRapid methodsPCRIMSIAC Acknowledgments This work was supported by a research grant from the Secretaria da Ciência e Tecnologia do Estado do Rio Grande do Sul (SCT no. 41/01). Author ANM was recipient of a scholarship from CAPES, Ministry of Education, Brazil.