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Artigo Produção Nacional Revisado por pares
BIBTEX RIS

Steady-state level of bone morphogenetic protein-15 in goat ovaries and its influence on in vitro development and survival of preantral follicles

2011; Elsevier BV; Volume: 338; Issue: 1-2 Linguagem: Inglês

10.1016/j.mce.2011.02.007

ISSN

1872-8057

Autores

J.J.H. Celestino, I.B. Lima-Verde, Jamily Bezerra Bruno, M.H.T. Matos, Roberta Nogueira Chaves, M. V. A. Saraiva, Cleidson Manoel Gomes da Silva, Luciana Rocha Faustino, R. Rossetto, C.A.P. Lopes, Mariana Aragão Matos Donato, Christina Alves Peixoto, C.C. Campello, J.R.V. Silva, J.R. Figueiredo,

Tópico(s)

Tissue Engineering and Regenerative Medicine

Resumo

This study investigates steady-state level of bone morphogenetic protein-15 (BMP-15) mRNA in caprine follicles, and the effects of BMP-15 on in vitro development of preantral follicles. Ovarian fragments were cultured for one or seven days in Minimal Essential Medium (MEM+) with BMP-15 (0, 1, 10, 50, 100 or 200 ng/mL), and further analyzed by histology, transmission electron and fluorescent microscopy. BMP-15 mRNA in secondary follicles was higher than in primordial and primary follicles. After seven days, 10, 50 or 100 ng/mL of BMP-15 maintained the percentage of normal follicles similar to the control (non-cultured), and increased the oocyte and follicle diameters when compared to the control and MEM+. BMP-15 at 100 ng/mL increased the secondary follicles and maintained their ultrastructural integrity. In conclusion, the BMP-15 mRNAs were detected in all follicular categories. BMP-15 (100 ng/mL) maintained the integrity and promoted the growth of caprine preantral follicles cultured for seven days.

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