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BIBTEX RIS

Selection of Single-Chain Antibodies against the VP8* Subunit of Rotavirus VP4 Outer Capsid Protein and Their Expression in Lactobacillus casei

2004; American Society for Microbiology; Volume: 70; Issue: 11 Linguagem: Inglês

10.1128/aem.70.11.6936-6939.2004

ISSN

1098-5336

Autores

Vicente Monedero, Jesús Rodríguez‐Díaz, Rosa Viana, Javier Buesa, Gaspar Pérez‐Martínez,

Tópico(s)

Viral Infectious Diseases and Gene Expression in Insects

Resumo

ABSTRACT Single-chain antibodies (scFv) recognizing the VP8* fraction of rotavirus outer capsid and blocking rotavirus infection in vitro were isolated by phage display. Vectors for the extracellular expression in Lactobacillus casei of one of the scFv were constructed. L. casei was able to secrete active scFv to the growth medium, showing the potential of probiotic bacteria to be engineered to express molecules suitable for in vivo antirotavirus therapies.

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