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Detection of B Epitopes on the p24 gag Protein of Feline Immunodeficiency Virus by Monoclonal Antibodies

1993; Mary Ann Liebert, Inc.; Volume: 9; Issue: 2 Linguagem: Inglês

10.1089/aid.1993.9.141

1931-8405

Stefania Lombardi, Mauro Bendinelli, Carlo Garzelli,

T-cell and B-cell Immunology

Seven monoclonal antibodies were obtained after immunization of mice with purified sodium dodecyl sulfate (SDS)-disrupted feline immunodeficiency virus (FIV). Six antibodies specifically bound antigens in the cytoplasm of FIV-infected cells as determined by indirect immunofluorescence and reacted with FIV p24 gag gene product in immunoblots. One reacted positively with virus-infected cells, but failed to recognize FIV structural proteins by immunoblotting. Using competition binding studies, the anti-p24 monoclonals were shown to detect four distinct B-cell epitopes. Competition with sera of FIV-infected cats showed that such epitopes are immunogenic also in the natural host species.