Purification of a Plasmodium berghei neutral endopeptidase and its localization in merozoite
1987; Elsevier BV; Volume: 26; Issue: 1-2 Linguagem: Inglês
10.1016/0166-6851(87)90140-x
ISSN1872-9428
AutoresFrançois Bernard, Joseph Schrével,
Tópico(s)Venomous Animal Envenomation and Studies
ResumoA Plasmodium berghei neutral endopeptidase specific for the fluorogenic substrates valyl-leucyl-glycyl-arginyl/lysyl-aminoethylcarbazole was purified by Fast Protein Liquid Chromatography. The enzyme was a Mr 68 000 polypeptide. Immunization of mice with the purified enzyme gave a specific antiserum, as demonstrated by immunoblotting. Immunofluorescence with this antiserum showed a strong labelling of P. berghei merozoites in mature segmented schizonts and of merozoites released from schizont-infected red blood cell. This labelling was mainly associated with the merozoite apex. It is possible that this endopeptidase is involved in the reinvasion.
Referência(s)