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Optimisation of reaction conditions for production ofS-(−)-2-hydroxypropiophenone byAcinetobacter calcoaceticus

1994; Springer Science+Business Media; Volume: 13; Issue: 5 Linguagem: Inglês

10.1007/bf01569730

0169-4146

Elizabeth Prosen, Owen P. Ward,

Microbial Metabolic Engineering and Bioproduction

Whole cells and cell-free extracts ofAcinetobacter calcoaceticus containing benzoylformate decarboxylase efficiently condensed benzoylformate and acetaldehyde to produce the acyloin compoundS-(−)-2-hydroxypropiophenone. Optimal concentrations of acetaldehyde cosubstrate for this reaction were found to be 1600 and 800 mM when whole cells and cell-free extracts were used respectively as biocatalysts. In both cases, optimal benzoylformate concentration was 100 mM. Temperature and pH optima for the biotransformation reaction were 30°C and 6.0 respectively. Under optimised conditions, maximum production of 2-hydroxypropiophenone, amounting to 8.4 g L−1, occurred after a 2-h incubation. Product formation equivalent to 6.95 g in 1 h corresponded to a productivity of 267 mg acyloin per g dry cells per h.