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CD11b expression on neutrophils in human crevicular fluid collected from clinically healthy gingivae

1991; Wiley; Volume: 26; Issue: 2 Linguagem: Inglês

10.1111/j.1600-0765.1991.tb01631.x

1600-0765

Keiko Watanabe, Karen L. Hagen, Burton R. Andersen,

Cell Adhesion Molecules Research

This study investigated the cell surface expression of CD11b (Mo 1, Mac‐1, CR 3) by neutrophils (PMNs) from human crevicular fluid (CF‐PMNs). CD11b expression on CF‐PMNs was compared, using flow cytometry, to that on peripheral blood PMNs (PB‐PMNs) isolated simultaneously from the same subjects. CD11b expression by CF‐PMNs was also compared to that on PBPMNs stimulated with formylmethionylleucylphenylalanine (fMLP) or crevicular fluid supernatant (cell‐free portion of CF diluted with buffer) (CFS). Crevicular PMNs consistently expressed more CD11b than unstimulated PB‐PMNs (p<0.001). The level of CD11b expressed on these CF‐PMNs was significantly greater than that expressed by PB‐PMNs stimulated with 10 −8 M fMLP (p<0.05). The level of CD11b surface expression on CF‐PMNs was not statistically different from that expressed on PB‐PMNs stimulated with CFS or 10 −6 M fMLP. However, CFS significantly stimulated upregulation of surface CDllb expression on PB‐PMNs relative to that achieved with 10 −8 M fMLP (0.01<p<0.025). Thus it is concluded that CF‐PMNs express a high level of CD1 Ib and CFS can upregulate CD11b expression on PB‐PMNs to a level that is at least as great as that achieved with 10 −8 M fMLP.