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Detection and quantitation of stanozolol (Stromba®) in urine by isotope dilution-mass fragmentography

1981; Pergamon Press; Volume: 14; Issue: 8 Linguagem: Inglês

10.1016/0022-4731(81)90007-8

1878-2353

Olle Lantto, Ingemar Björkhem, Håkan Ek, David B. Johnston,

Analytical Methods in Pharmaceuticals

A highly specific and sensitive method for the analysis of stanozolol (Stromba®) in urine, based on isotope dilution-mass spectrometry, has been developed. A fixed amount of deuterium labelled stanozolol was added to a fixed quantity of urine and the mixture was treated with Helix pomatia digestive juice. The hydrolysate was extracted with diethyl ether under alkaline conditions and the extract subjected to preparative thin-layer chromatography. The purified extract was treated with propylene oxide in order to alkylate the secondary amine in the heterocyclic ring. The material was then converted into the TMS ether derivative and subjected to gas chromatography-mass spectrometry. Unlabelled stanozolol was quantitated by selected monitoring of the ions at m/e 414 and at m/e 417. Under the conditions employed, stanozolol could be detected and quantified above a level of about 2 ng/ml of urine. After a single dose of 7.5–10 mg to human volunteers, stanozolol could be detected in urine for the next 2–3 days. The method had about the same sensitivity as the radioimmunoassay method commonly used and should be of value for the detection of the inappropriate use of the drug by athletes.