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NOTES ON THE CULTIVATION OF TREPONEMA PALLIDUM

1915; Rockefeller University Press; Volume: 21; Issue: 3 Linguagem: Inglês

10.1084/jem.21.3.213

1540-9538

Hans Zinsser, J. G. Hopkins, Ruth Gilbert,

Science and Education Research

We consider themost importantcontribution reported in this paper the fact that Treponema pallidum can be cultivated in fluid media, without the addition of agar, together with tissues sterilized by heat. This forms an excellent method of obtaining mass cultures for luetin preparation and immunological experimentation. We may add that while the tissue varieties employed have all stongly favored the growth of the treponemata, we have noticed especially active and motile cultures when lung and suprarenal tissues were employed.