Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Ban AI a new isoschizomer of the type II restriction endonuclease Hae III discovered in a Bacillus anthracis isolate from Amazon Basin

2002; Oxford University Press; Volume: 215; Issue: 1 Linguagem: Inglês

10.1111/j.1574-6968.2002.tb11376.x

1574-6968

Jocelei Maria Chies, Ana Christina de Oliveira Dias, Hélio Mauro Moreira Maia, Spartaco Astolfi‐Filho,

Advanced biosensing and bioanalysis techniques

Bacillus anthracis was isolated and identified from a bacterial collection of samples from the Amazon river bank. Type II restriction endonuclease activity was detected in this prokaryote, the enzyme was purified, the molecular mass of the native protein estimated by gel filtration, and optima pH, temperature and salt requirements were determined. Quality control assays showed complete absence of 'non-specific nucleases'. Restriction cleavage analysis and DNA sequencing of restriction fragments allowed unequivocal demonstration of 5'-GG downward arrow CC-3' as the recognition sequence. This enzyme was named BanAI and is therefore an isoschizomer of the prototype restriction endonuclease HaeIII. This is the first report of a type II restriction endonuclease identified, purified from a natural isolate of B. anthracis.