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Microwave-assisted extraction of bioactive alkaloids from Stephania sinica

2014; Elsevier BV; Volume: 130; Linguagem: Inglês

10.1016/j.seppur.2014.04.026

1873-3794

Dao-Tao Xie, Ya-Qin Wang, Yun Kang, Qiu-Fen Hu, Na-Ya Su, Jian Huang, Chun Tao, Ji Guo,

Natural product bioactivities and synthesis

A microwave-assisted extraction method was developed for the extraction of four bioactive alkaloids from Stephania sinica, and the quantification of the alkaloids was achieved by HPLC. The factors of major effects on the extraction were selected by single factor experiments, and the optimal levels of three main effective factors were obtained using the response surface method by employing Box–Behnken design. The optimal conditions for maximizing the extraction yields of the alkaloids were determined as follows: liquid-to-solid ratio 24:1 mL/g, ethanol concentration 65%, extraction time 90 s, temperature 60 °C, and microwave power 150 W. In comparison with the Soxhlet and ultrasonic-assisted extractions, the proposed method was fast, energy-saving and environmental-friendly.