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Tailored noise waveform/collision‐induced dissociation of ions stored in a linear ion trap combined with liquid chromatography/Fourier transform ion cyclotron resonance mass spectrometry

2004; Wiley; Volume: 18; Issue: 22 Linguagem: Inglês

10.1002/rcm.1664

1097-0231

Andrey N. Vilkov, Bogdan Bogdanov, Ljiljana Paša‐Tolić, Dave C. Prior, Gordon Anderson, Christophe Masselon, Ronald J. Moore, Richard Smith,

Advanced Proteomics Techniques and Applications

Abstract A new collision‐induced dissociation (CID) technique based on broadband tailored noise waveform (TNW) excitation of ions stored in a linear ion trap has been developed. In comparison with the conventional sustained off‐resonance irradiation (SORI) CID method commonly used in Fourier transform ion cyclotron resonance mass spectrometry (FTICR‐MS), this MS/MS technique increases throughput by eliminating the long pump‐down delay associated with gas introduction into the high vacuum ICR cell region. In addition, the TNW‐CID method speeds spectrum acquisition since it does not require Fourier transformation, calculation of resonant frequencies and generation of the excitation waveforms. We demonstrate TNW‐CID coupled with on‐line capillary reverse‐phase liquid chromatography separations for the identification of peptides. The experimental results are compared with data obtained using conventional quadrupole ion trap MS/MS and SORI‐CID MS/MS in an ICR cell. Copyright © 2004 John Wiley & Sons, Ltd.