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In vivo biosynthesis of the antifreeze protein in the winter flounder — Evidence for a larger precursor

1978; Elsevier BV; Volume: 85; Issue: 1 Linguagem: Inglês

10.1016/s0006-291x(78)80059-x

1090-2104

C. L. Hew, Nelson Liunardo, Garth L. Fletcher,

Physiological and biochemical adaptations

Biosynthesis of the winter flounder's antifreeze protein was examined by the in vivo incorporation of tritiated alanine into 10% trichloroacetic acid-soluble serum proteins. Two radioactive components of 15,000 and 10,000 daltons were observed. The latter species corresponded to authentic flounder antifreeze protein. Pulse chase experiments, tryptic cleavage and amino acid analysis of the 15,000 daltons component suggest flounder antifreeze protein is synthesized via the larger protein.