The use of carrier RNA to enhance DNA extraction from microfluidic-based silica monoliths
2009; Elsevier BV; Volume: 652; Issue: 1-2 Linguagem: Inglês
10.1016/j.aca.2009.03.038
ISSN1873-4324
AutoresKirsty J. Shaw, Lauren Thain, Peter Docker, Charlotte E. Dyer, John Greenman, Gillian M. Greenway, Stephen J. Haswell,
Tópico(s)Microfluidic and Bio-sensing Technologies
ResumoDNA extraction was carried out on silica-based monoliths within a microfluidic device. Solid-phase DNA extraction methodology was applied in which the DNA binds to silica in the presence of a chaotropic salt, such as guanidine hydrochloride, and is eluted in a low ionic strength solution, such as water. The addition of poly-A carrier RNA to the chaotropic salt solution resulted in a marked increase in the effective amount of DNA that could be recovered (25 ng) compared to the absence of RNA (5 ng) using the silica-based monolith. These findings confirm that techniques utilising nucleic acid carrier molecules can enhance DNA extraction methodologies in microfluidic applications.
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