Atomic force microscope imaging of chromosome structure during G-banding treatments
1997; NRC Research Press; Volume: 40; Issue: 1 Linguagem: Inglês
10.1139/g97-018
ISSN1480-3321
AutoresAntonio Musio, I Sbrana, Tullio Mariani, C. Frediani, C. Ascoli,
Tópico(s)RNA and protein synthesis mechanisms
ResumoSurface topography of human chromosomes was examined by atomic force microscopy during treatments for G-banding. Trypsin treatment resulted in a structural modification in the chromatin. Subsequent Giemsa staining caused a general swelling of the chromosomal surface that was greater in the areas of G-band positive regions. By means of a quantitative evaluation method we showed that the G-banding process produces a 10-fold enhancement of a pre-existing pattern of chromatin between G-band positive and G-band negative regions on mitotic chromosomes.Key words: atomic force microscopy, human chromosomes, G-banding, trypsin treatment, Giemsa stain.
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