HPLC-APCI-MS with calibration based on stable isotope-labelled internal standards for the quantification of carbonyls in air samples
1999; Royal Society of Chemistry; Volume: 124; Issue: 9 Linguagem: Inglês
10.1039/a904393d
ISSN1364-5528
AutoresGabriela Zurek, Uwe Kärst, Heinrich Luftmann,
Tópico(s)Mass Spectrometry Techniques and Applications
ResumoA method for the selective determination of formaldehyde and acetaldehyde in air samples based on HPLC-APCI-MS with calibration using stable isotope-labelled standards was developed. d2-Formaldehyde-2,4-dinitrophenylhydrazone and d4-acetaldehyde-2,4-dinitrophenylhydrazone were synthesized from 2,4-dinitrophenylhydrazine and the respective deuterated aldehydes. The labelled derivatives were used as internal calibration standards in the quantification of formaldehyde and acetaldehyde in complex matrices, including automobile exhaust. Co-elution of the labelled and non-labelled derivatives in HPLC provides for identical mass spectrometric conditions, thus significantly reducing the relative standard deviation of the internal calibration approach compared with external calibration with non-labelled derivatives.
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