Fluorescent quantum dot-labeled aptamer bioprobes specifically targeting mouse liver cancer cells
2009; Elsevier BV; Volume: 81; Issue: 1-2 Linguagem: Inglês
10.1016/j.talanta.2009.12.031
ISSN1873-3573
AutoresJing Zhang, Xing Jia, Xiaojing Lv, Yulin Deng, Hai‐Yan Xie,
Tópico(s)Advanced Biosensing Techniques and Applications
ResumoFluorophore-labeled bioprobes are the key for fluorescent-labeled imaging technology. In the present work, mouse liver hepatoma cell line BNL 1ME A.7R.1 (MEAR)-specific ssDNA aptamer TLS9a was used to fabricate quantum dot-labeled aptamer bioprobe (QD-Apt), which was obtained by conjugating streptavidin-modified quantum dots (SA-QDs) with biotin-derived aptamer via the interaction between biotin and streptavidin. The QD-Apt was of monodispersity and excellent fluorescence properties. When the optimum ratio of SA-QDs to aptamer, which is 1:16, was used in the preparation of the QD-Apt, the resultant QD-Apt was of satisfactory bioactivity. They could specifically recognize MEAR cells and could not recognize BNL cells and Hela cells. Particularly, the growth and viability of QD-Apt bound MEAR cells were not affected by QD-Apt within 84 h compared to control cells, indicating that the probe was biocompatible and suitable for live cell imaging.
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