Isolation and characterization of a antimicrobial compound from bacillus coagulans
2008; Taylor & Francis; Volume: 12; Issue: 1 Linguagem: Inglês
10.1080/19768354.2008.9647152
ISSN2151-2485
Autores Tópico(s)Enzyme Production and Characterization
ResumoAbstract A bacterium strain called Bacillus coagulans was isolated from an industrial wastewater drainage and selected for its antimicrobial activities against bacteria and fungi. Characterization studies strongly suggested that this strain is Bacillus coagulans. Antimicrobial activity was found against gram‐positive, gram‐negative bacteria and yeast strain. Maximal activity was observed after 24 h when incubated at 30°C and pH 8. The activity was found to be stable at 75°C for 30 min and at pH range of 2–12. Analysis of the antimicrobial compound by SDS‐PAGE suggested a molecular mass of approximately 7.5 KDa. The substance was characterized as a bacteriocin, because of its proteinaceous nature and low molecular weight. Our bacteriocin could potentially be used as a food preservative, because of its thermostable property and broad antimicrobial spectrum. Key words: Antimicrobial activity Bacillus coagulans BacteriocinFood preservative Notes To whom correspondence should be addressed. Tel:+82–2–2220–0954; Fax: +82–2–2220–4474 E‐mail: emadm_abada@yahoo.co.uk
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