Reactive oxygen species are involved in the activation of cellular phospholipase A 2
1992; Wiley; Volume: 309; Issue: 2 Linguagem: Inglês
10.1016/0014-5793(92)81092-z
ISSN1873-3468
AutoresRalph F. Goldman, E. Ferber, U. Zort,
Tópico(s)Vitamin K Research Studies
ResumoVanadate (V) potentiated (4‐ to 10‐fold) the activation of cellular phospholipase A 2 (PLA 2 ) induced by H2O2 (H), a phorbol ester (T), a Ca 2+ ‐ionophore (A) and opsonized zymosan in macrophages. V+H induced in intact cells the activation and translocation of PLA 2 and protein kinase C(PKC) to the plasma membrane. V+H and V+T+A induced strong chemiluminescence (CL) which was abrogated by a specific NADPH oxidase inhibitor diphenylene iodonium (DPI). DPI markedly suppressed the stimulation of PLA 2 by V+T+A and V+OZ. The results suggest that the formation of endogenous reactive oxygen species (ROS) is important for PLA 2 activation.
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