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BIBTEX RIS

Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system

2014; Nature Portfolio; Volume: 4; Issue: 1 Linguagem: Inglês

10.1038/srep03943

ISSN

2045-2322

Autores

Yicheng Zhao, Zhen Dai, Liang Huai Yang, Minghao Yin, Kuiying Ma, Mei He, Hongsheng Ouyang, Chun‐Bo Teng,

Tópico(s)

Advanced biosensing and bioanalysis techniques

Resumo

Here, we report a convenient and efficient miRNA inhibition strategy employing the CRISPR system. Using specifically designed gRNAs, miRNA gene has been cut at a single site by Cas9, resulting in knockdown of the miRNA in murine cells. Using a modified CRISPR interference system (CRISPRi), inactive Cas9 can reversibly prevent the expression of both monocistronic miRNAs and polycistronic miRNA clusters. Furthermore, CRISPR/CRISPRi is also capable of suppressing genes in porcine cells.

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