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Kinetics of toluene degradation by a nitrate-reducing bacterium isolated from a groundwater aquifer

1997; Wiley; Volume: 55; Issue: 1 Linguagem: Inglês

10.1002/(sici)1097-0290(19970705)55

1097-0290

Joacim Elmén, Wubin Pan, Shuk-yu Leung, Andrew C. Magyarosy, Jay D. Keasling,

Microbial Fuel Cells and Bioremediation

Biotechnology and BioengineeringVolume 55, Issue 1 p. 82-90 Kinetics of toluene degradation by a nitrate-reducing bacterium isolated from a groundwater aquifer Joacim Elmén, Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778Search for more papers by this authorWubin Pan, Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778Search for more papers by this authorSo Yan Leung, Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778Search for more papers by this authorAndrew Magyarosy, Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778Search for more papers by this authorJ. D. Keasling, Corresponding Author keasling@socrates.berkeley.edu Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778;Search for more papers by this author Joacim Elmén, Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778Search for more papers by this authorWubin Pan, Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778Search for more papers by this authorSo Yan Leung, Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778Search for more papers by this authorAndrew Magyarosy, Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778Search for more papers by this authorJ. D. Keasling, Corresponding Author keasling@socrates.berkeley.edu Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778Department of Chemical Engineering, University of California, Berkeley, California 94720-1462; telephone (510) 642-4862; fax: (510) 642-4778;Search for more papers by this author First published: 26 March 2000 https://doi.org/10.1002/(SICI)1097-0290(19970705)55:1 3.0.CO;2-5Citations: 13AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinked InRedditWechat Abstract Groundwater from a xylene-contaminated acquifer was enriched in the laboratory in the presence of toluene, xylenes, ethylbenzene, and benzene. A pure culture that degrades toluene and m-xylene under nitrate-reducing conditions was isolated. Fatty acid analysis, 16S rRNA sequencing, and morphological traits indicate that the isolate was a strain of Azoarcus tolulyticus. The kinetics of toluene degradation under nitrate-reducing conditions by this isolate was determined. Nitrate reduction does not proceed beyond nitrite. Nitrate and toluene are substrate limiting at low concentrations, whereas toluene, nitrate, and nitrite are inhibitory at high concentrations. Several inhibition models were compared to experimental data to represent inhibition by these substrates. A kinetic model for toluene and nitrate degradation as well as for cell growth and nitrite production was developed and compared to experimental data. The results of this work may find important application in the remediation of groundwater aquifers contaminated with aromatic hydrocarbons. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 82–90, 1997. Citing Literature Volume55, Issue15 July 1997Pages 82-90 RelatedInformation