Produção Nacional
Revisado por pares
Leishmania chagasi: lipophosphoglycan characterization and binding to the midgut of the sand fly vector Lutzomyia longipalpis
2002; Elsevier BV; Volume: 121; Issue: 2 Linguagem: Inglês
10.1016/s0166-6851(02)00033-6
ISSN1872-9428
AutoresRodrigo Pedro Soares, Maria Eduarda Figueiredo Macedo, Catherine Ropert, Nelder F. Gontijo, Igor C. Almeida, Ricardo T. Gazzinelli, Paulo F. P. Pimenta, Salvatore J. Turco,
Tópico(s)Helminth infection and control
ResumoDuring metacyclogenesis of Leishmania in its sand fly vector, the parasite differentiates from a noninfective, procyclic form to an infective, metacyclic form, a process characterized by morphological changes of the parasite and also biochemical transformations in its major surface lipophosphoglycan (LPG). This glycoconjugate is polymorphic among species with variations in sugars that branch off the conserved Gal(β1,4)Man(α1)-PO4 backbone of repeat units and the oligosaccharide cap. LPG has been implicated as an adhesion molecule that mediates the interaction with the midgut epithelium of the sand fly. These adaptations were explored in the context of the structure and function of LPG for the first time on a New World species, Leishmania chagasi. The distinguishing feature of LPG of procyclic L. chagasi consisted of β1,3-glucose residues that branch off the disaccharide-phosphate repeat units and also are present in the cap. Importantly, metacyclic L. chagasi significantly down-regulate the glucose substitutions in the LPG. The significance of these modifications was demonstrated in the interaction of L. chagasi with its vector Lutzomyia longipalpis. In contrast to procyclic parasites and procyclic LPG, metacyclic parasites and metacyclic LPG were unable to bind to the insect midgut. These results are consistent with the proposal that a New World Leishmania species, similar to Old World species, adapts the expression of terminally exposed sugars of its LPG to mediate parasite–sand fly interactions.
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