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Pineal modulation of ACTH 1–17 effect upon murine corticosterone production

1983; Elsevier BV; Volume: 11; Issue: 1 Linguagem: Inglês

10.1016/0361-9230(83)90060-6

1873-2747

Salvador Sáchez De La Pen̄a, Franz Halberg, E Halberg, Frank Ungar, Germaine Cornélissen, Elizabeth Sánchez, Gregory M. Brown, Lawrence E. Scheving, Edmond G. Yunis, Paul Vecsei,

Adrenal Hormones and Disorders

In tests of Corticosterone production in vitro, aqueous pineal homogenate (APH) modulates the effect of a short-chain ACTH analogue, ACTH 1–17, added to adrenals from different circadian stages. Adrenal and pineal glands from female B6D2F1 mice, standardized on staggered LD 12:12 regimens, were obtained at the same clock-hour from each room, in order to cover 6 different circadian stages. Adrenals from each circadian stage were bisected and incubated with APH from the same circadian stage (isophasic incubation) or from one of the other 5 circadian stages (heterophasic incubation). ACTH 1–17 (0.05 IU) was added to each incubation medium. After 4 hours of incubation at 37°C with 95% O2 and 5% CO2, the media were stored at −20°C until Corticosterone RIA were done. APH was found to have a statistically significant modulatory effect upon the stimulation by ACTH 1–17 of adrenal Corticosterone production in vitro. This APH effect changed rhythmically as a function of circadian stage from amplification over no effect to attenuation, as a so-called feed-sideward.