Portal de Periódicos da CAPES

Phosphoenolpyruvate carboxykinase in the adult liver fluke, Fasciola hepatica

1968; Pergamon Press; Volume: 24; Issue: 3 Linguagem: Inglês

10.1016/0010-406x(68)90789-5

1879-3029

Roger K. Prichard, Philip J. Schofield,

Parasite Biology and Host Interactions

1. Fasciola hepatica incorporates C14O2 via an active phosphoenolpyruvate carboxykinase. 2. Phosphoenolpyruvate carboxykinase activity is associated with both the soluble and mitochondrial fractions. 3. Maximum activity occurred in the mitochondrial fraction at pH 5·9. 4. The activity of the phosphoenolpyruvate carboxykinase was dependent on the presence of a nulceotide and divalent cation. IDP was the most effective nucleotide, but high activity was demonstrated with either ADP or GDP. Mg2+ was much less effective than Mn2+. 5. The role of phosphoenolpyruvate carboxykinase as possibly the key enzyme linking glycolysis with the production of C4-dicarboxylic acids in F. hepatica is discussed.