Portal de Periódicos da CAPES

Controlling electroosmotic flow in capillary zone electrophoresis

1994; Elsevier BV; Volume: 678; Issue: 1 Linguagem: Inglês

10.1016/0021-9673(94)87086-1

1873-3778

Nava Cohen, Eli Grushka,

Electrowetting and Microfluidic Technologies

Electroosmotic flow in capillary zone electrophoresis must be controlled for precise and reproducible performance. We found that the addition of any one of several classes of compounds such as amines, amino acids or organic acids to the running buffer stabilizes electroosmotic flow. With the additives in the buffer, the precision of migration times improved to better than 1%. The reproducibilities improved to better than 5%. The system is very stable at various conditions for small molecules and for proteins at very acidic or very basic conditions.