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Direct-Write Patterning of Bacterial Cells by Dip-Pen Nanolithography

2012; American Chemical Society; Volume: 134; Issue: 40 Linguagem: Inglês

10.1021/ja3073808

1943-2984

Jieun Kim, Younghun Shin, Seong‐Hun Yun, Dong-Sik Choi, Jihye Nam, Sung Ryong Kim, Sung‐Kwon Moon, Bong Hyun Chung, Jae-hyuck Lee, Jae‐Ho Kim, Kiyoung Kim, Kyung‐Min Kim, Jung‐Hyurk Lim,

Monoclonal and Polyclonal Antibodies Research

The ability of dip-pen nanolithography (DPN) to generate nano- or microarrays of soft or hard materials (e.g., small molecules, DNA, proteins, nanoparticles, sols, and polymers) in a direct-write manner has been widely demonstrated. The transporting of large-sized ink materials such as bacteria, however, remains a significant challenge with this technique. The size limitation of the water meniscus formed between the DPN tip and the solid surface becomes a bottleneck in such diffusion-based molecular transport experiments. Herein, we report a straightforward "stamp-on" DPN method that uses a nanostructured poly(2-methyl-2-oxazoline) hydrogel-coated tip and carrier agents to generate patterns of micrometer-sized Escherichia coli JM 109 bacterial cells. We demonstrate that this approach enables the deposition of a single bacterial cell array on a solid surface or arrays of layers of multiple cells by modulating the viscosity of the "ink" solution. Fluorescence microscopy images indicated that the deposited bacterial cells were kept alive on Luria–Bertani-agar layered solid surfaces after DPN patterning.