Producing selenomethionine-labeled proteins with a baculovirus expression vector system
1999; Elsevier BV; Volume: 7; Issue: 11 Linguagem: Inglês
10.1016/s0969-2126(00)80020-9
ISSN1878-4186
AutoresJohn J. Bellizzi, Joanne Widom, Christopher W. Kemp, Jon Clardy,
Tópico(s)Selenium in Biological Systems
ResumoBoth selenomethionine (SeMet) based multiwavelength anomalous diffraction (MAD) phasing and protein production in baculovirus expression vector systems have greatly enhanced the scope and speed of macromolecular crystallography, but the apparent incompatibility of these two powerful techniques presents an obstacle to many structure determinations. The use of either technique alone is essentially routine, and although their individual use is growing dramatically (Figure 1), their combined use is virtually unknown. As of 14th July 1999, the Protein Data Bank contained no deposited coordinates for structures solved using MAD phasing on baculovirus produced SeMet-labeled proteins.
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