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A high-throughput test to detect C.E.R.A. doping in blood

2009; Elsevier BV; Volume: 50; Issue: 5 Linguagem: Inglês

10.1016/j.jpba.2009.06.038

1873-264X

Séverine Lamon, Sylvain Giraud, Léonie Egli, Jessica Smolander, Michael Jarsch, Kay‐Gunnar Stubenrauch, Alice Hellwig, Martial Saugy, Neil Robinson,

Hormonal and reproductive studies

C.E.R.A., a continuous erythropoietin receptor activator, is a new third-generation erythropoiesis-stimulating agent (ESA) that has recently been linked with abuse in endurance sports. In order to combat this new form of doping, we examined an enzyme-linked immunosorbent assay (ELISA) designed to detect the presence of C.E.R.A. in serum samples. The performance of the assay was evaluated using a pilot excretion study that involved six subjects receiving C.E.R.A. Validation data demonstrated an excellent reproducibility and ensured the applicability of the assay for anti-doping purposes. To maximize the chances of detecting the drug in serum samples, we propose the use of this specific ELISA test as a high-throughput screening method, combined with a classic isoelectric focusing test as a confirmatory assay. This strategy should make C.E.R.A. abuse relatively easy to detect, thereby preventing the future use of this drug as a doping agent.