Prostaglandin release from in vitro guinea-pig gallbladder
1983; Elsevier BV; Volume: 25; Issue: 2 Linguagem: Inglês
10.1016/0090-6980(83)90100-4
ISSN1878-416X
AutoresMichael L. Booker, Wayne W. LaMorte,
Tópico(s)Peptidase Inhibition and Analysis
ResumoIn order to study prostaglandin in release from guinea pig gallbladder, full thickness tissue sections were incubated for one hour in Kreb's solution. Extraction and two dimensional chromatography of incubation media obtained in the presence of radio-labelled arachidonic acid demonstrated the presence of PGE2, PGF2α, 6-keto-PGF1α and thromboxane B2. These results were supported by radioimmunoassay of incubations conducted in the absence of exogenous arachidonate and in the presence of varying concentrations of unlabelled exogenous arachidonate. The previously reported predominance of PGE2 was only seen at high concentrations of exogenous arachidonate.
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