Antimicrobial Profile of Selected Snake Venoms and Their Associated Enzymatic Activities
2012; Volume: 2; Issue: 4 Linguagem: Inglês
10.9734/bmrj/2012/2091
ISSN2231-0886
Autores Tópico(s)Amphibian and Reptile Biology
ResumoAims: To investigate the antiviral and antibacterial profile of several crude snake venoms and to assess some of their enzymatic activities.Methodology: The antiviral activities of Naja haje, Bitis arietans, Naja nigricollis and Echis carinatus snake venoms were investigated against Herpes simplex virus type1, Rift valley fever virus and Vesicular stomatitis virus using the end point of cytopathic effect method.Antibacterial activities of Bitis arietans, Cerastes cerastes, Echis carinatus, Vipera lebetina, Naja naja, Pseudechis australis, Naja nigricollis and Naja haje venoms were examined against Staphylococcus aureus, Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa using disc diffusion method.Microdilution method was used to determine the venom's minimum inhibitory concentration.L-amino acid oxidase and phospholipase A2 activities of crude venoms were evaluated using enzymatic assays.Results: Naja nigricollis, Bitis arietans and Echis carinatus snake venoms exhibited significant antiviral activities against all test viruses, except for N. haje treated cells.The mean depletion of viral infectivity titer of venom pretreated cells was higher than its depletion post viral infection for all three venoms showing antiviral activities.Naja nigricollis exhibited the highest antiviral activity against test viruses and recorded a mean depletion of viral infectivity titer in venom pretreated cells of 3.8 log (10) / ml , 3.2 log (10) / ml and 2.5 Research Article
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