Cajal body-specific small nuclear RNAs: a novel class of 2'-O-methylation and pseudouridylation guide RNAs
2002; Springer Nature; Volume: 21; Issue: 11 Linguagem: Inglês
10.1093/emboj/21.11.2746
ISSN1460-2075
AutoresXavier Darzacq, Beáta E. Jády, Céline Verheggen, Arnold M. Kiss, Édouard Bertrand, Tamás Kiss,
Tópico(s)Neurogenetic and Muscular Disorders Research
ResumoArticle3 June 2002free access Cajal body-specific small nuclear RNAs: a novel class of 2′-O-methylation and pseudouridylation guide RNAs Xavier Darzacq Xavier Darzacq Laboratoire de Biologie Moléculaire Eucaryote du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, Cedex, France Search for more papers by this author Beáta E. Jády Beáta E. Jády Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Search for more papers by this author Céline Verheggen Céline Verheggen Institut de Génétique Moléculaire, Montpellier, France Search for more papers by this author Arnold M. Kiss Arnold M. Kiss Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Search for more papers by this author Edouard Bertrand Edouard Bertrand Institut de Génétique Moléculaire, Montpellier, France Search for more papers by this author Tamás Kiss Corresponding Author Tamás Kiss Laboratoire de Biologie Moléculaire Eucaryote du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, Cedex, France Search for more papers by this author Xavier Darzacq Xavier Darzacq Laboratoire de Biologie Moléculaire Eucaryote du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, Cedex, France Search for more papers by this author Beáta E. Jády Beáta E. Jády Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Search for more papers by this author Céline Verheggen Céline Verheggen Institut de Génétique Moléculaire, Montpellier, France Search for more papers by this author Arnold M. Kiss Arnold M. Kiss Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary Search for more papers by this author Edouard Bertrand Edouard Bertrand Institut de Génétique Moléculaire, Montpellier, France Search for more papers by this author Tamás Kiss Corresponding Author Tamás Kiss Laboratoire de Biologie Moléculaire Eucaryote du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, Cedex, France Search for more papers by this author Author Information Xavier Darzacq1, Beáta E. Jády2, Céline Verheggen3, Arnold M. Kiss2, Edouard Bertrand3 and Tamás Kiss 1 1Laboratoire de Biologie Moléculaire Eucaryote du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse, Cedex, France 2Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary 3Institut de Génétique Moléculaire, Montpellier, France *Corresponding author. E-mail: [email protected] The EMBO Journal (2002)21:2746-2756https://doi.org/10.1093/emboj/21.11.2746 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Cajal (coiled) bodies are conserved subnuclear organelles that are present in the nucleoplasm of both animal and plant cells. Although Cajal bodies were first described nearly 100 years ago, their function has remained largely speculative. Here, we describe a novel class of human small nuclear RNAs that localize specifically to Cajal bodies. The small Cajal body- specific RNAs (scaRNAs) are predicted or have already been demonstrated to function as guide RNAs in site-specific synthesis of 2′-O-ribose-methylated nucleotides and pseudouridines in the RNA polymerase II-transcribed U1, U2, U4 and U5 spliceosomal small nuclear RNAs (snRNAs). Our results provide strong support for the idea that the Cajal body, this mysterious nuclear organelle, provides the cellular locale for post-transcriptional modification of spliceosomal snRNAs. Introduction In eukaryotic cells, biogenesis of cytoplasmic mRNAs, tRNAs, rRNAs, small nuclear RNAs (snRNAs) and nucleolar RNAs (snoRNAs) takes place in the nucleus. The interphase nucleus contains at least a dozen morphologically distinct subdomains, called nuclear bodies, which are believed to function in the synthesis, processing, modification or transport of various cellular RNAs. However, with the exception of the nucleolus, which has long been known as the site of the synthesis and maturation of cytoplasmic rRNAs, the precise function of nuclear bodies remained largely elusive (Lamond and Earnshaw, 1998; Matera, 1999; Lewis and Tollervey, 2000). Besides the nucleolus, the Cajal (coiled) body is the most prominent and most extensively studied nucleoplasmic organelle (Matera, 1998; Gall, 2000). Cajal bodies are enriched with spliceosomal and nucleolar ribonucleoproteins (snRNPs and snoRNPs) and components of the basal transcription machinery. But, despite the rapidly growing list of its molecular components, the precise function of the Cajal body is still unknown. The removal of intronic sequences of precursor mRNAs (pre-mRNAs) is catalysed by the spliceosome, a dynamic assembly of five snRNAs and numerous protein factors. The U1, U2, U4, U5 and U6 spliceosomal snRNAs play a fundamental role in pre-mRNA splicing: they select the correct splice sites and orchestrate formation of the active spliceosome (Burge et al., 1999; Will and Lührmann, 2001). The spliceosomal snRNAs contain many post-transcriptionally synthesized 2′-O-ribose-methylated nucleotides and pseudouridines (Reddy and Busch, 1988; Massenet et al., 1998). These modified nucleotides are confined to the functionally important regions of snRNAs, which are known to interact with pre-mRNAs, other snRNAs or spliceosomal proteins. Most likely, the modified nucleotides facilitate formation of the specific and dynamic molecular interactions required for the correct function of the spliceosome. Consistent with this view, modification of the U2 snRNA is fundamental for the assembly of the active spliceosome (Pan and Prives, 1989; Ségault et al., 1995; Yu et al., 1998). The mechanism responsible for the site-specific synthesis of modified nucleotides in snRNAs has long remained an enigma. Recently, it has been established that the nucleolus contains an enormous number of snoRNAs that direct 2′-O-methylation and pseudouridylation of rRNAs (Smith and Steitz, 1997; Kiss, 2001). The 2′-O-methylation guide snoRNAs carry the conserved 5′-terminal C (RUGAUGA) and 3′-terminal D (CUGA) boxes as well as imperfect internal copies of these elements, called C′ and D′ boxes. To select the correct methylation site, sequences preceding the D or D′ box of the snoRNA form a 10–21 bp helix with rRNAs (Cavaillé et al., 1996; Kiss-László et al., 1996). The pseudouridylation guide snoRNAs fold into a consensus ‘hairpin–hinge–hairpin–tail’ structure and contain the conserved H (ANANNA) and ACA boxes (Balakin et al., 1996; Ganot et al., 1997b). They possess bipartite rRNA recognition motifs, which form two short helices with ribosomal sequences preceding and following the substrate uridine (Ganot et al., 1997a; Ni et al., 1997). The conserved box C/D and H/ACA motifs play an essential role in snoRNA-guided RNA modification reactions as well as in the processing and nucleolar accumulation of snoRNAs. The C/D and H/ACA boxes accomplish these jobs through binding of specific snoRNP proteins. Thus far, four box C/D (fibrillarin, Nop56p, Nop58p and Snu13p) and four box H/ACA (Gar1p, dyskerin, Nhp2p and Nop10p) snoRNA-associated proteins have been identified (Kiss, 2001). Most likely, fibrillarin catalyses the box C/D snoRNA-guided 2′-O-methylation reaction (Tollervey et al., 1993; Wang et al., 2000) and dyskerin is the pseudouridine synthase in box H/ACA snoRNPs (Lafontaine et al., 1998; Zebarjadian et al., 1999). Recent evidence indicates that synthesis of the eight 2′-O-methylated nucleotides and the three pseudouridines in the RNA polymerase (pol) III-synthesized U6 spliceosomal snRNA takes place in the nucleolus and that it is directed by box C/D and H/ACA snoRNAs (Tycowski et al., 1998; Ganot et al., 1999). The U6 snRNA, which is transcribed in the nucleoplasm, is transported to the nucleolus to undergo snoRNA-mediated modification (Lange and Gerbi, 2000). Less is known about modification of the pol II-transcribed U1, U2, U4 and U5 snRNAs. Following nucleoplasmic synthesis, these RNAs are transported to the cytoplasm to associate with seven Sm proteins and undergo cap hypermethylation and 3′-end formation (Will and Lührmann, 2001). The newly assembled snRNPs are re-imported to the nucleoplasm, where they accumulate in interchromatin granule clusters (Lamond and Ernshow, 1998; Sleeman and Lamond, 1999b; Matera, 1999; Lewis and Tollervey, 2000). However, a fraction of snRNPs accumulates transiently in Cajal bodies (Carmo-Fonseca et al., 1992; Matera and Ward, 1993; Spector 1993; Sleeman and Lamond, 1999a) and, under certain conditions, in the nucleolus (Lyon et al., 1997; Sleeman and Lamond, 1999a; Lange and Gerbi, 2000; Yu et al., 2001), raising the possibility that these nuclear bodies might have a function in the biogenesis, trafficking or storage of snRNPs. Microinjection experiments demonstrated that internal modification of the U2 snRNA occurs in the nucleus rather than in the cytoplasm of Xenopus oocytes (Yu et al., 2001). The same experiments favoured the nucleolus as the intranuclear locale for U2 modification. This conclusion was seemingly supported by identification of the U85 box C/D–H/ACA composite guide RNA that functions in both 2′-O-methylation and pseudouridylation of the U5 snRNA (Jády and Kiss, 2001). In this study, we have characterized six novel putative guide RNAs that are predicted to direct 2′-O-methylation and pseudouridylation of the RNA pol II-specific U1, U2, U4 and U5 snRNAs. Unexpectedly, the new guide RNAs as well as the previously characterized U85 RNA accumulate specifically in Cajal bodies and therefore they comprise a novel class of small nuclear RNAs, called the small Cajal body-specific RNAs (scaRNAs). These results indicate that the Cajal body functions in the post-transcriptional modification of pol II-specific spliceosomal snRNAs. Results Identification of novel composite box C/D–H/ACA RNAs We have recently characterized an unusual modification guide RNA, the U85 RNA, which functions both in 2′-O-methylation and pseudouridylation of the U5 snRNA (Jády and Kiss, 2001). The U85 RNA is composed of a box C/D and a box H/ACA snoRNA-like domain (Figure 1), and is associated with both fibrillarin and Gar1p, which are specific protein components of box C/D and box H/ACA snoRNPs, respectively. To identify novel box C/D–H/ACA composite RNAs, human HeLa RNAs were immunoprecipitated with anti-fibrillarin antibody and size-fractionated on a sequencing gel. RNAs migrating between labelled DNA markers of 234 and 368 nucleotides were recovered and used as a template for cDNA synthesis (Kiss-László et al., 1996). In brief, the 5′ and 3′ termini of the purified RNAs were tagged with a phosphorylated oligonucleotide by using T4 RNA ligase. The double-tagged RNAs were amplified by RT–PCR and cloned into a plasmid vector. Sequence analyses of several individual clones identified three putative snoRNAs, called U87, U88 and U89, which possessed C and D box motifs, but otherwise lacked significant similarity to any known RNAs (Figure 1). Northern blot analyses confirmed that the new RNAs are expressed in HeLa cells (data not shown). The genomic copies of the new RNAs were found within introns of putative protein-coding genes, indicating that they are generated by intron processing (see legend to Figure 1). Figure 1.Computer-predicted two-dimensional structures of human box C/D–H/ACA snoRNAs. The evolutionarily conserved box C, D, H, ACA and the putative box C′ and D′ sequence motifs are boxed. The structure of the U85 snoRNA has been adopted from Jády and Kiss (2001). The genomic copies of the U85 and U89 snoRNAs are in the fourth and sixth introns of the structural maintenance of chromatin (D63880) and the B cell-associated protein (U75511) genes, respectively. The U87 and U88 snoRNAs are encoded within the ninth and twelfth introns of a putative protein-coding gene (BC000061). Download figure Download PowerPoint A computer-aided structural analysis revealed that the U87, U88 and U89 RNAs can be folded into a two-dimensional structure that is highly reminiscent of the architecture of the U85 box C/D–H/ACA RNA characterized previously (Figure 1). In each RNA, the 5′- and 3′-terminal regions carrying the C and D boxes can form a long stem structure, whereas the middle region folds into the consensus ‘hairpin–hinge–hairpin–tail’ structure of box H/ACA snoRNAs. Moreover, the single-stranded hinge and tail regions carry potential box H and ACA sequences, respectively. To assess whether the novel putative box C/D–H/ACA snoRNAs are associated with both box C/D and H/ACA snoRNP proteins, immunoprecipitation experiments were performed from a human HeLa cell extract using anti-fibrillarin and anti-GAR1 antibodies (Figure 2A). RNase A/T1 protection analyses demonstrated that the U87, U88 and U89 RNAs, like the U85 box C/D–H/ACA RNA, were precipitated by both antibodies. Please notice that immunological properties of the human U90 and U92 RNPs, although tested in this experiment, will be discussed later. As expected, the U19 box H/ACA snoRNP was precipitated only by the anti-GAR1 and the U3 box C/D snoRNP was recognized only by the anti-fibrillarin antibody and neither of the two antibodies reacted with the U4 spliceosomal snRNP. We concluded that the newly identified U87, U88 and U89 RNAs, together with U85, comprise a new group of snoRNAs that are composed of a box C/D and a H/ACA snoRNA domain, and are associated with both box C/D and box H/ACA snoRNP proteins. Figure 2.Characterization of the novel RNAs. (A) Immunoprecipitation of human snoRNPs. An extract prepared from HeLa cells was incubated with protein A–Sepharose saturated with anti-fibrillarin (α-Fib) or anti-GAR1 (α-Gar) antibodies. Upon collection of Sepharose beads by centrifugation, RNAs were recovered by proteinase K treatment and phenol extraction. Distribution of RNAs was determined by RNase A/T1 mapping using sequence-specific antisense RNA probes as indicated on the right. Control mappings performed with E.coli tRNA (C) or RNAs obtained from the HeLa cell extract (E) are also shown. Mock represents control reaction with protein A–Sepharose alone. Lane M, size markers (terminally labelled HaeIII- and TaqI-digested pBR322). (B) Intracellular localization. RNA isolated either from HeLa cells (T), or from nuclear (Nu), nucleoplasmic (Np), nucleolar (No) or cytoplasmic (Cy) fractions of HeLa cells were analysed by RNase A/T1 mapping using sequence-specific RNA probes. Lane C represents control mapping with E.coli tRNA. Download figure Download PowerPoint Box C/D–H/ACA RNAs can direct post-transcriptional modification of pol II-specific spliceosomal snRNAs Examination of the sequences of the new box C/D–H/ACA RNAs showed that they lack significant sequence complementarity to rRNA sequences, making it unlikely that they function in rRNA modification. We have recently demonstrated that the human U85 box C/D–H/ACA RNA directs 2′-O-methylation of the C45 and pseudouridylation of the U46 residues in the U5 spliceosomal snRNA (Figure 3). We therefore investigated whether the novel box C/D–H/ACA RNAs could also function in snRNA modification. We found that sequences preceding the D and the putative D′ boxes of the U87 RNA could, in principle, direct 2′-O-methylation of the A65 and U41 residues in the U4 and U5 snRNAs, respectively (Figure 3). Indeed, both of these nucleotides are known to be 2′-O-methylated in vertebrate U4 and U5 snRNAs (Reddy and Busch, 1988; Massenet et al., 1998). Like U87, the U88 RNA was also predicted to function in 2′-O-methylation of the U41 residue in the U5 snRNA, although apart from the putative U5 recognition elements, the two RNAs show no significant sequence similarity. Likewise, the U89 RNA and the previously characterized U85 RNA also seem to share a common function, namely directing pseudouridylation of the U46 residue in the U5 snRNA. Again, the U85 and U89 RNAs, disregarding the short U5 recognition motifs, possess no overall sequence similarity. Our observations strongly suggest that the box C/D–H/ACA RNAs function in the modification of pol II-specific spliceosomal snRNAs and that synthesis of 2′-O-methylated nucleotides and pseudouridines in snRNAs is frequently achieved by functionally redundant snoRNPs. Figure 3.Potential base-pairing interactions between human box C/D–H/ACA RNAs and spliceosomal snRNAs. Nucleotides predicted to be selected for 2′-O-methylation (closed circle) or pseudouridylation (Ψ) in the human snRNAs are indicated. The 5′-terminal hairpins of the box H/ACA snoRNA-like domains of the U85 and U89 RNAs are schematically represented. Positions of the H, D and D′ boxes are indicated. Download figure Download PowerPoint Human U85 RNA accumulates in Cajal bodies So far, all small RNAs carrying the box C/D or H/ACA motifs have been found to accumulate in the nucleolus (Smith and Steitz, 1997; Tollervey and Kiss, 1997; Weinstein and Steitz, 1999). In fact, the conserved C/D and H/ACA boxes are the key determinants of the nucleolar targeting of snoRNAs (Lange et al., 1998; Samarsky et al., 1998; Narayanan et al., 1999a, b). Therefore, the U85, U87, U88 and U89 box C/D–H/ACA RNAs were assumed to accumulate in the nucleolus. However, during exploration of cDNA libraries of human nucleoplasmic or nucleolar RNAs, we noticed that partial or full-length cDNAs of the U85 RNA frequently appeared in the nucleoplasmic library, but were not found in the nucleolar one (our unpublished results). To test the significance of this unexpected observation, human HeLa cells were fractionated into nuclear, nucleoplasmic, nucleolar and cytoplasmic fractions, and distribution of the U85 RNA was determined by RNase A/T1 mapping (Figure 2B). To our surprise, the U85 RNA was found mostly in the nucleoplasmic fraction. As expected, the U19 box H/ACA and the U3 box C/D snoRNAs showed a predominantly nucleolar accumulation, while the U4 spliceosomal snRNA appeared mostly in the nucleoplasmic fraction. The fascinating discovery that the U85 box C/D–H/ACA RNA co-purifies with the nucleoplasmic fraction of HeLa cells prompted us to examine further the localization of this RNA using fluorescent in situ hybridization microscopy (Figure 4A). Probing of HeLa cells with a U85-specific fluorescent antisense RNA revealed that the U85 RNA, instead of showing a uniform distribution, localizes to a few sharp, dot-like structures in the nucleoplasm. Since this distribution pattern of U85 was highly reminiscent of that of Cajal bodies (Matera, 1998; Gall, 2000), the cells were also stained with an antibody directed against p80 coilin, a protein marker of the Cajal body (Andrade et al., 1991). The U85 RNA and p80 coilin co-localized perfectly, demonstrating that the nucleoplasmic dots revealed by the U85 probe correspond to Cajal bodies. Figure 4.Human U85 box C/D–H/ACA RNA localizes to Cajal bodies. (A) In situ localization of the endogenous U85 RNA. Human HeLa cells were probed with a fluorescent antisense RNA complementary to the human U85 RNA from position 1 to 76 and with an antibody directed against p80 coilin as indicated above the panels. The merged image shows that the U85 RNA co-localizes with p80 coilin. Scale bar, 10 μm. (B) In situ localization of transiently overexpressed U85 and mgU6-53 RNAs in HeLa cells. The schematic structure of the pCMV-globin expression construct is shown. The exons (E1, E2 and E3) and the polyadenylation site (PA) of the human β-globin gene are indicated. The transcription initiation site of the cytomegalovirus promoter (CMV) is indicated. The coding regions of the human U85 and mgU6-53 RNAs in the second intron of the globin gene are represented by an open arrow. Relevant restriction sites are shown (H, HindIII; C, ClaI; X, XhoI). The pCMV-globin-mgU6-53 plasmid was co-transfected with an expression construct producing a GFP-tagged version of the human fibrillarin protein. The overexpressed U85 and mgU6-53 RNAs were visualized by sequence-specific fluorescent RNA probes and Cajal bodies were specifically stained by anti-p80 coilin. Arrows indicate nucleolar stained structures enriched in mgU6-53 and fibrillarin. Arrowheads point to Cajal bodies, which clearly contain mgU6-53, fibrillarin and p80 coilin. The contour of the nucleus of a non-transfected HeLa cell is highlighted. Download figure Download PowerPoint To exclude the possibility that a fraction of U85 RNA accumulates outside the Cajal bodies, we investigated the distribution of U85 RNA after overproduction in HeLa cells. To this end, the human U85 RNA gene was inserted into the second intron of the human β-globin gene, which had been placed under the control of the cytomegalovirus (CMV) promoter (Figure 4B). The human U85 RNA was efficiently and correctly expressed in human HeLa and simian COS-7 cells transfected with the pCMV-globin-U85 expression construct (Jády and Kiss, 2001; data not shown). The in situ labelling patterns of the U85 RNA probe and the p80 coilin antibody were compared in transfected HeLa cells (Figure 4B). Upon hybridization with the U85 fluorescent probe, a strong signal was observed in cells that overproduced the U85 RNA. With the same exposure time, endogenous U85 RNA was not detected in the nucleus of non-transfected cells (Figure 4B, circled). Double labelling with anti-p80 coilin clearly showed that even after its overproduction, the U85 RNA accumulated exclusively in Cajal bodies. Since RNase A/T1 mappings failed to detect 5′- and/or 3′-extended precursors of U85 (Jády and Kiss, 2001; data not shown), we concluded that the U85-specific sequences accumulating in the Cajal bodies represent fully processed U85 RNAs. As a control, the human mgU6-53 box C/D snoRNA, which directs 2′-O-methylation of the RNA pol III-transcribed U6 spliceosomal snRNA (Ganot et al., 1999), was also overproduced in HeLa cells. In situ hybridization revealed that the mgU6-53 snoRNA accumulated in large areas of the nucleus, as well as in small dots. It clearly co-localized with a green fluorescent protein (GFP)-tagged version of human fibrillarin, an abundant nucleolar protein that is also present in Cajal bodies (Gall, 2000). Therefore, the mgU6-53 snoRNA accumulated mainly in the nucleolus of transfected cells and, to a smaller extent, in the Cajal bodies, as confirmed by staining with the anti-coilin antibody. This observation was fully consistent with our previous cell fractionation experiments, in which the mgU6-53 snoRNA was localized mainly to the nucleolar fraction of human HeLa cells (Ganot et al., 1999). In summary, we concluded that in both transfected and non-transfected HeLa cells, mature U85 RNA localizes specifically to Cajal bodies. Box C/D–H/ACA RNAs reside in Cajal bodies We then investigated the subcellular localization of the newly discovered box C/D–H/ACA RNAs. Cell fractionation experiments revealed that the U87, U88 and U89 RNAs, like U85, accumulate mostly in the nucleoplasmic fraction of HeLa cells (Figure 2B). To facilitate in situ localization, the U87, U88 and U89 RNAs were overexpressed in HeLa and COS-7 cells using the pCMV-globin expression vector as described for the U85 RNA. RNase A/T1 mappings demonstrated that the U87, U88, and U89 RNAs were efficiently and faithfully expressed in transfected HeLa and COS-7 cells (data not shown). To determine the localization of the overproduced U87, U88 and U89 RNAs in HeLa cells, double labelling experiments were carried out with sequence-specific fluorescent RNA (U87, U89) or oligonucleotide (U88) probes and the anti-p80 coilin antibody (Figure 5). For each RNA, a perfect co-localization was observed with coilin, demonstrating that the overexpressed RNAs accumulate in the Cajal bodies of HeLa cells. The same results were obtained by in situ localization of the transiently expressed human U87, U88 and U89 RNAs in COS-7 cells (data not shown). Moreover, probing of non-transfected HeLa cells with the U88 probe specifically stained Cajal bodies, indicating that endogenous U88 RNA also resides in this nuclear organelle. Our results demonstrate that the human U85, U87, U88 and U89 box C/D–H/ACA RNAs comprise a novel class of cellular RNAs that localize specifically to the Cajal body. The new group of snRNAs was collectively named small Cajal body-specific RNAs (scaRNAs). Figure 5.In situ localization of human U87, U88 and U89 box C/D–H/ACA RNAs. Human U87, U88 and U89 RNAs were transiently over expressed in HeLa cells using the pCMV-globin expression construct. The intracellular distribution of the overproduced U87, U88 and U89 RNAs as well as the endogenous HeLa U88 RNA (U88endo) was investigated by fluorescent in situ hybridization. Cajal bodies were visualized by staining with anti-p80 coilin antibody. For other details, see the legend to Figure 4. Download figure Download PowerPoint Guide RNAs implicated in modification of pol II-specific spliceosomal snRNAs reside in Cajal bodies The results presented thus far indicate that scaRNAs directing modification of snRNAs are composed of a box C/D and a H/ACA snoRNA domain. In cDNA libraries of human fibrillarin- and Gar1p-associated snoRNAs, we have identified three novel RNAs, called U90, U91 and U92 (Figure 6A). The U90 and U91 RNAs possess the characteristic core motif of box C/D snoRNAs, which includes the conserved C and D boxes and a short 5′, 3′-terminal helix. Immunoprecipitation experiments demonstrated that the U90 (Figure 2A) and U91 (data not shown) RNAs represent bona fide fibrillarin-associated box C/D snoRNAs and are not associated with the Gar1 box H/ACA snoRNP protein. The U90 and U91 RNAs also carry putative C′ and D′ boxes, which are juxtaposed by short internal helices. This indicates that they probably function in RNA 2′-O-methylation, since positioning the C′ and D′ boxes in close proximity to each other is essential for the box D′-dependent 2′-O-methylation reaction (Kiss-László et al., 1998). Indeed, we found that sequences preceding the D′ boxes of U90 and U91 can position the A70 and C8 residues in the human U1 and U4 snRNAs, respectively, for 2′-O-methylation (Figure 6A). Both of these nucleotides are in fact 2′-O-methylated in mammalian U1 and U4 snRNAs (Reddy and Busch, 1988). In contrast to U90 and U91, the human U92 RNA lacks C and D boxes, but it folds into the consensus ‘hairpin–hinge–hairpin–tail’ structure of box H/ACA snoRNAs (Figure 6A). The U92 RNA binds the Gar1, but not the fibrillarin snoRNP protein (Figure 3A), demonstrating that it is an authentic box H/ACA snoRNA. We noticed that the U92 box H/ACA RNA, in theory, is capable of directing pseurouridylation of the U44 residue in the U2 snRNA (Figure 6A). During the course of this study, a large-scale characterization of mouse non-coding nuclear RNAs revealed partial sequences of the apparent mouse orthologues of the human U91 (MBII-119) and U92 (MBI-57) RNAs, showing that these putative snRNA modification guide RNAs are conserved in mammalian cells (Hüttenhofer et al., 2001). Figure 6.Localization of box C/D and H/ACA RNAs predicted to function in modification of pol II-specific spliceosomal snRNAs. (A) Structure and function of human U90, U91 box C/D and U92 box H/ACA RNAs. The conserved box C/D and the potential C′ and D′ motifs of the U90 and U91 RNAs are indicated. Inverted arrows under the sequences of U90 and U91 indicate nucleotides predicted to form terminal and internal helices. The predicted two-dimensional structure of the U92 RNA was obtained by computer folding. The H and ACA sequence motifs are boxed. Predicted base-pairing interactions of the U90, U91 and U92 RNAs with the U1, U4 and U2 snRNAs, respectively, are indicated. The 2′-O-methylated A70 and C8 nucleotides in the selected sequences of the U1 and U4 snRNAs are indicated by dots. The pseudouridine residue (Ψ) at position 44 in the human U2 snRNA is marked. (B) Localization of human U90, U91 and U92 RNAs in HeLa cells. Overexpressed (U90 and U92) and endogenous (U91endo and U92endo) modification guide RNAs were visualized with sequence-specific fluorescent probes. For other details, see legends to Figures 4 and 5. Download figure Download PowerPoint To assess whether all guide RNAs implicated in modification of pol II-specific snRNAs accumulate in Cajal bodies, we investigated the intracellular localization of the newly identified U90, U91 and U92 RNAs. Encouragingly, cell fractionation experiments revealed a nucleoplasmic localization for the U90 box C/D and the U92 box H/ACA RNAs (Figure 2B). Next, probing of HeLa cells with fluorescent antisense probes specific for the U91 and U92 RNAs resulted in strongly staining dots in the nucleoplasm (Figure 6B, U91endo and U92endo). Double labelling with anti-p80 coilin antibody showed that the dots highlighted by the U91- and U92-specific probes corresponded to Cajal bodies. The U92 RNA, probably due to its low abundance, was not directly detectable in HeLa cells. Therefore, localization of this RNA and also the U90 RNA was investigated in HeLa cells transfected with pCMV-globin constructs expressing the U92 and U90 RNAs. U90 and U92 were correctly and efficiently expressed in transfected HeLa cells (data not shown) and, as in situ localization experiments demonstrated, localized specifically to Cajal bodies (Figure 6B, U90 and U92). We concluded that 2′-O-methylation and pseudouridylation guide RNAs that function in post-transcriptional modification of the RNA pol II-transcribed spliceosomal snRNAs, irrespective of their box elements, associated proteins and molecular sizes, specifically and exclusively accumulate i
Referência(s)