Development and Validation of an Oligonucleotide Microarray for Detection of Multiple Virulence and Antimicrobial Resistance Genes in Escherichia coli
2006; American Society for Microbiology; Volume: 72; Issue: 5 Linguagem: Inglês
10.1128/aem.72.5.3780-3784.2006
ISSN1098-5336
AutoresGuillaume Bruant, Christine Maynard, Sadjia Békal, Isabelle Gaucher, Luke Masson, Roland Brousseau, Josée Harel,
Tópico(s)Antibiotic Resistance in Bacteria
ResumoABSTRACT An oligonucleotide microarray detecting 189 Escherichia coli virulence genes or markers and 30 antimicrobial resistance genes was designed and validated using DNA from known reference strains. This microarray was confirmed to be a powerful diagnostic tool for monitoring emerging E. coli pathotypes and antimicrobial resistance, as well as for environmental, epidemiological, and phylogenetic studies including the evaluation of genome plasticity.
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